|Life Sciences - Life Science Article|
|Written by Manoj Pandey|
|Monday, 18 May 2009|
A REVIEW ON MICROBIOLOGIAL ASSAY
Microbiological assays are designed to perform the qualitative and quantitative estimations of specific viable microorganisms present in the samples. The test should be performed in all the samples to ensure that the it is free from any micro-organism and it also ensure that it will not going to harm the human body as concern to the micro-organism.
The whole 10 pages article is available for download at Downloads section of Farmavita.Net
Microbiological assay are designed to perform the qualitative and quantitative estimations of specific viable microorganisms present in the samples. It includes tests for total viable count (bacteria and fungi) and specified microbial species (Escherichia coli, Salmonellla, Pseudomonas aeruginosa and Staphylococcus aureus). It must be carried out under conditions designed to avoid accidental microbial contamination of the preparation during the test. When the test specimens have antimicrobial activity or contain antimicrobial substances must be eliminated by means of procedure such as dilution, filtration, neutrilization or inactivation. In performing the test, precautions must be taken to prevent biohazard. According to USP the test is designed to determine total aerobic microbial count and yeast and mould count. This test demonstrates that product is free from Staphylococcus aureus, E. coli, Pseudomonas aeruginosa, C. albicans and A. niger.
PREPARTION OF THE TEST SOLUTION
Phosphate Buffer (pH 7.2), buffered Sodium Chloride-Peptone Solution or Fluid medium used for the test is used to dissolve or dilute the specimen.
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